Checkpoint Controls and Cancer: Volume 2: Activation and Regulation Protocols (Methods in Molecular Biology)

Checkpoint Controls and Cancer: Volume 2: Activation and Regulation Protocols (Methods in Molecular Biology)

* Publisher: Humana Press; 1 edition (June 24, 2004)
* Language: English
* ISBN-10: 1588295001
* ISBN-13: 978-1588295002
Book Description

Prestigious researchers from ten different countries present readily reproducible experimental protocols to study the molecular components of checkpoint controls and their regulation. Described in step-by-step detail, these cutting-edge techniques offer novel approaches, such as the use of genome databases and siRNA, to analyzing how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype. An earlier first volume, Reviews and Model Systems, comprehensively reviews the complexities of checkpoint controls and the model systems needed to study them.

Download
Rapidshare

Human Embryonic Stem Cell Protocols (Methods in Molecular Biology)

Human Embryonic Stem Cell Protocols (Methods in Molecular Biology)
Humana Press | ISBN 1588294978 | 2006-03-01 | PDF | 8.1 MB | 408 pages
A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download
Easy-share

Robin, Ed. Harris, "Cell Biology Protocols "

Robin, Ed. Harris, "Cell Biology Protocols "
John Wiley & Sons; 1 edition (2006) | ISBN:0470847581 | 402 pages | PDF | 13 Mb

As a modern composite scientific discipline, Cell Biology has expanded and moved forward rapidly in recent years. Cell Biologists now require a wide range of techniques, including those of analytical biochemistry and microscopy in all its diverse forms. These are often used alongside the techniques of molecular biology and molecular genetics. This book contains numerous useful protocols, covering light and electron microscopy, cell culture, cell separation, subcellular fractionation, organelle and membrane isolation, and the use of in vitro reassembly systems in Cell Biology. Many of these protocols feature helpful notes and safety information for practical application. The format favours easy use at the bench with space for notes and important safety information. An appendix contains essential analytical information that will prove invaluable to those working on all aspects of cell biology.

This book will be of interest to students and more experienced cell biologists, as well as molecular biologists and those working in genomics and proteomics who are looking for cellular techniques to validate their findings within intact cells.
Download
Rapidshare
Morror

Plant Proteomics: Methods and Protocols (Methods in Molecular Biology, Vol 355)

Plant Proteomics: Methods and Protocols (Methods in Molecular Biology, Vol 355)
Humana Press | 1588296350 | Edition - 2007-10-28 | PDF | 399 pages | 4.21MB | Rapidshare & Megaupload

Plant Proteomics: Methods and Protocols provides up-to-date methods and protocols for plant proteomics, each written by expert researchers from leading laboratories. Experimental methodologies are explained in clear and complete detail for both the wet and dry laboratories. The methods and protocols presented in this volume will inspire the novice as well as the expert researcher to undertake new experimental endeavors in plant proteomics.

Chapters 1 and 2 describe standard protocols and chapters 3 through 10 contain optimized protocols for protein extraction of tissues such as cereal seeds, xylem, phloem sap, of woody plants, and of cellular compartments, such as chloroplasts and mitochondria. These methods rely on improvements to solubilization of proteins, which is the emphasis of Chapters 11 and 12. Chapters 13 to 23 outline methods for protein identification, including 2DLC or LC of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D gels, blue native ID gels, and Edman sequencing. Chapters 24 to 29 focus on the rapidly expanding area of analysis of post-translational modifications and protein-protein relationships. Attention is also given to data that proteomics produces, including management of that data, and to statistical tools used for effective analysis.

Download

Rapidshare

Megaupload

Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology Volume 9)

Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology Volume 9)
Humana Press | 0896035646 | Edition - 2000-03-31 | PDF | 240 pages | 1.5 MB | rapidshare

Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology

Download

Rapidshare
FileFactory

Food Microbiology Protocols (Methods in Biotechnology)

John F. T. Spencer, Alicia L. Ragout de Spencer, "Food Microbiology Protocols (Methods in Biotechnology)"
Humana Press; 1st edition | ISBN: 089603867X | 512 pages | PDF | 3,4 Mb
Expert laboratorians present a wide ranging set of detailed techniques for investigating the nature, products, and extent of the microorganisms involved in the manufacture and spoilage of foodstuff. The methods cover pathogenic organisms that cause spoilage, microorganisms in fermented foods, and microorganisms producing metabolites that affect the flavor or nutritive value of foods. Included in the section dealing with fermented foods are procedures for the maintenance of lactic acid bacteria, the isolation of plasmid and genomic DNA from species Lactobacillus, and the determination of proteolytic activity of lactic acid bacteria. A substantial number of chapters are devoted to yeasts, their use in food and beverage production, and techniques for improving industrially important strains. Comprehensive and timely, Food Microbiology Protocols is a gold-standard collection of readily reproducible techniques essential for the study of the wide variety of microorganisms involved in food production, quality, storage, and preservation today.

Download
Rapidshare
Mirror1
Mirror2

PRINS and In Situ PCR Protocols (Methods in Molecular Biology: Volume 334)

PRINS and In Situ PCR Protocols (Methods in Molecular Biology: Volume 334)
Humana Press | 1588295494 | Edition - 2006-04-01 | PDF | 264 pages | 2.2 MB | rapidshare

This completely revised and updated edition takes advantage of the many new developments and applications that have occurred in PRINS and in situ PCR technology, including a new fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutation analysis, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. The authors describe in detail various applications of PRINS in human (detection of gene deletions in cancer detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus, and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation.

Download

Rapidshare

Megaupload

Microchip Capillary Electrophoresis: Methods And Protocols (Methods in Molecular Biology: Volume 339)
Humana Press | ISBN 1588292932 | Edition - 2006-03-01 | PDF | 237 pages | 2.4 MB | rapidshare

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Download

Rapidshare

Megaupload

Yawen, Ed. Bai, "Protein Folding Protocols "

Yawen, Ed. Bai, "Protein Folding Protocols "
Humana Press; 1 edition (2007) | ISBN: 1588296229 | 327 pages | PDF | 3,6 Mb

Protein Folding Protocols presents protocols for studying and characterizing protein folding from the unfolded to the folded state. Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a broad range of techniques to study, predict, and analyze the protein folding process.
Protein Folding Protocols also provides sample approaches toward the prediction of protein structure starting from the amino acid sequence, in the absence of overall homologous sequences. These approaches have tremendous implications, ranging from drug design, functional assignment, comprehension of the nature of regulation, understanding molecular machines, viral entry into cells, and putting together cellular pathways and their dynamics.
The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Download
Depositfiles

Macromolecular Cyrstallography Protocols Vol.2: Structure Determination (Methods in Molecular Biology, Vol 364)

Macromolecular Cyrstallography Protocols Vol.2: Structure Determination (Methods in Molecular Biology, Vol 364)
Humana Press | 1588299023 | Edition - 2007 | PDF | 283 pages | 3.71 MB | Rapidshare & Megaupload

In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

Download

Rapidshare

or

Megaupload

Sylvie, Ed. Doublie, "Macromolecular Cyrstallography Protocols V.2: Structure Determination"

Sylvie, Ed. Doublie, "Macromolecular Cyrstallography Protocols V.2: Structure Determination"
Humana Press; 1 edition (2007) | ISBN: 1588299023 | 283 pages | PDF | 3,5 Mb

In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

Download
Depositfiles

Enzymes in Nonaqueous Solvents: Methods and Protocols (Methods in Biotechnology Volume 15)

Enzymes in Nonaqueous Solvents: Methods and Protocols (Methods in Biotechnology Volume 15)
Humana Press | ISBN:0896039293 | Edition - 2001-01-15 | PDF | 704 pages | 3.21 MB | Rapidshare & Aliceinwire

In Enzymes in Nonaqueous Solvents: Methods and Protocols, leading chemists, biochemists, biotechnologists, and process engineers summarize for the first time a wide range of methods for executing enzymatic transformations under nonaqueous conditions. Each method includes detailed step-by-step instructions for its successful completion, a list of materials, and ancillary notes that explain the scientific basis of the procedure, as well as troubleshooting. Also provided are a generic description of key reactions, advice on biocatalyst preparation, discussion of reaction conditions, and instructions on bioreactor design.
Comprehensive and state-of-the-art, Enzymes in Nonaqueous Solvents: Methods and Protocols offers today's synthetic chemists, biochemists, and process engineers all the essential information needed to carry out enzymatic reactions in nonaqueous media, as well as to successfully scale up to production quantities.

Download

Rapidshare

Aliceinwire

Alicenwire registry

A Manual for Biochemistry Protocols (Manuals in Biomedical Research)

Markus R. Wenk, Aaron Z. Fernandis, "A Manual for Biochemistry Protocols (Manuals in Biomedical Research)"
World Scientific Publishing Company; 1 Spi edition (March 30, 2007) | ISBN: 9812700668 | 140 pages | PDF | 1,5 Mb

Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements.

Download
Depositfiles
Rapidshare

Handbook of Detection of Enzymes on Electrophoretic Gels, Second Edition

Handbook of Detection of Enzymes on Electrophoretic Gels, Second Edition
CRC | ISBN 0849312574 | 2002-12-23 | PDF | 3.1 MB | 592 pages
Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date. New in the Second Edition: · An overview of new applications of enzyme electrophoresis and zymogram techniques · Zymogram techniques for approximately 100 enzymes not included in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes · Information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms · An appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels. The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology.

Download

Easy-share

A Manual for Biochemistry Protocols (Manuals in Biomedical Research)

Markus R. Wenk, Aaron Z. Fernandis, "A Manual for Biochemistry Protocols (Manuals in Biomedical Research)"
World Scientific Publishing Company; 1 Spi edition (March 30, 2007) | ISBN: 9812700668 | 140 pages | PDF | 1,5 Mb

Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols. This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements.

Download
Rapidshare
depositfiles